Journal: iScience

Article Title: DNA methylation-based profiling of horse archaeological remains for age-at-death and castration

doi: 10.1016/j.isci.2023.106144

Figure Lengend Snippet:

Article Snippet: N-Lauroylsarcosine solution 30% 500 mL , Dutscher , N-Lauroylsarcosine solution 30% 500 mL.

Techniques: Recombinant, Ligation, Purification, Software

Journal: Pharmaceutics

Article Title: Pneumococcal Surface Protein A-Hybrid Nanoparticles Protect Mice from Lethal Challenge after Mucosal Immunization Targeting the Lungs

doi: 10.3390/pharmaceutics14061238

Figure Lengend Snippet: Factors investigated for NP optimization.

Article Snippet: Carboxymethyl chitosan (CM-CS) (degree of deacetylation 80–95%, MW 30–500 KDa) and chitosan hydrochloride (HCl-CS) (degree of deacetylation 80–95%, MW 30–200 KDa) were purchased from Heppe Medical Chitosan GmbH, Halle, Germany.

Techniques:

Journal: Pharmaceutics

Article Title: Pneumococcal Surface Protein A-Hybrid Nanoparticles Protect Mice from Lethal Challenge after Mucosal Immunization Targeting the Lungs

doi: 10.3390/pharmaceutics14061238

Figure Lengend Snippet: Effect of chitosan hydrochloride or carboxymethyl chitosan addition on particle size, poly dispersity index (PDI) and zeta-potential of hybrid NPs prepared by o / w single emulsion method. ( a ) particle size, ( b ) PDI, and ( c ) zeta-potential of PGA-co-PDL and PLGA hybrid nanoparticles. Data represent mean ± SD, n = 3.

Article Snippet: Carboxymethyl chitosan (CM-CS) (degree of deacetylation 80–95%, MW 30–500 KDa) and chitosan hydrochloride (HCl-CS) (degree of deacetylation 80–95%, MW 30–200 KDa) were purchased from Heppe Medical Chitosan GmbH, Halle, Germany.

Techniques: Zeta Potential Analyzer, Emulsion

Journal: Pharmaceutics

Article Title: Pneumococcal Surface Protein A-Hybrid Nanoparticles Protect Mice from Lethal Challenge after Mucosal Immunization Targeting the Lungs

doi: 10.3390/pharmaceutics14061238

Figure Lengend Snippet: Effect of chitosan hydrochloride or carboxymethyl chitosan addition on particle size, zeta-potential, and poly dispersity index (PDI) of hybrid NPs formulated by the w / o / w double emulsion method (mean ± SD, n = 3).

Article Snippet: Carboxymethyl chitosan (CM-CS) (degree of deacetylation 80–95%, MW 30–500 KDa) and chitosan hydrochloride (HCl-CS) (degree of deacetylation 80–95%, MW 30–200 KDa) were purchased from Heppe Medical Chitosan GmbH, Halle, Germany.

Techniques: Double Emulsion

Journal: Pharmaceutics

Article Title: Pneumococcal Surface Protein A-Hybrid Nanoparticles Protect Mice from Lethal Challenge after Mucosal Immunization Targeting the Lungs

doi: 10.3390/pharmaceutics14061238

Figure Lengend Snippet: In vitro release of pneumococcal surface protein A (PspA4Pro) from NPs in phosphate buffered saline, pH 7.4. ( a ) PGA-co-PDL and ( b ) PLGA NPs carrying surface-adsorbed PspA4Pro at pH 4 or 7 prepared by o / w single emulsion. ( c ) PGA-co-PDL and ( d ) PLGA NPs prepared at pH 7.0 by w / o / w double emulsion with encapsulated PspA4Pro. The inset in ( d ) shows the same data in another Y-axis scale. Hybrid NPs were prepared with carboxymethyl chitosan (CM-CS) and with chitosan hydrochloride (HCl-CS). Data represent mean ± SD, n = 3.

Article Snippet: Carboxymethyl chitosan (CM-CS) (degree of deacetylation 80–95%, MW 30–500 KDa) and chitosan hydrochloride (HCl-CS) (degree of deacetylation 80–95%, MW 30–200 KDa) were purchased from Heppe Medical Chitosan GmbH, Halle, Germany.

Techniques: In Vitro, Saline, Emulsion, Double Emulsion

Journal: Pharmaceutics

Article Title: Pneumococcal Surface Protein A-Hybrid Nanoparticles Protect Mice from Lethal Challenge after Mucosal Immunization Targeting the Lungs

doi: 10.3390/pharmaceutics14061238

Figure Lengend Snippet: Recognition of PspA4Pro released from NPs by anti-PspA antibodies. ( a ) PGA-co-PDL and ( c ) PLGA NPs with or without chitosan prepared at pH 4.0 or 7.0 by o / w single emulsion with surface-adsorbed PspA4Pro. ( b ) PGA-co-PDL and ( d ) PLGA NPs prepared at pH 7.0 by w / o / w double emulsion with encapsulated PspA4Pro. Chitosan hydrochloride (HCl-CS) or carboxymethyl chitosan (CM-CS) were employed. PspA4Pro was used as a positive control. Data represent mean ± SD, n = 3.

Article Snippet: Carboxymethyl chitosan (CM-CS) (degree of deacetylation 80–95%, MW 30–500 KDa) and chitosan hydrochloride (HCl-CS) (degree of deacetylation 80–95%, MW 30–200 KDa) were purchased from Heppe Medical Chitosan GmbH, Halle, Germany.

Techniques: Emulsion, Double Emulsion, Positive Control

Journal: Pharmaceutics

Article Title: Pneumococcal Surface Protein A-Hybrid Nanoparticles Protect Mice from Lethal Challenge after Mucosal Immunization Targeting the Lungs

doi: 10.3390/pharmaceutics14061238

Figure Lengend Snippet: Lactoferrin binding assay of PspA4Pro released from NPs prepared with or without chitosan carrying surface-adsorbed PspA4Pro at pH 4.0 or 7.0 and NPs prepared at pH 7.0 by water-oil-water double emulsion with encapsulated PspA4Pro. ( a ) PGA-co-PDL NPs. ( b ) PLGA NPs. Chitosan hydrochloride (HCl-CS) or carboxymethyl chitosan (CM-CS) were employed. PspA4Pro standard was used as positive control. Data represent mean ± SD, n = 3. Statistical analysis: ANOVA/Tukey’s comparison test, * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: Carboxymethyl chitosan (CM-CS) (degree of deacetylation 80–95%, MW 30–500 KDa) and chitosan hydrochloride (HCl-CS) (degree of deacetylation 80–95%, MW 30–200 KDa) were purchased from Heppe Medical Chitosan GmbH, Halle, Germany.

Techniques: Binding Assay, Double Emulsion, Positive Control, Comparison